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Dear candidates you will find MCQ questions of Recombinant DNA Technology here. Learn these questions and prepare yourself for coming examinations and interviews. You can check the right answer of any question by clicking on any option or by clicking view answer button.
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Q. 1) Phage T7 promoter containing plasmids are used for over-expression of cloned genes because
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Q. 2) Though the right border (RB) and left border (LB) of T-DNA are identical, the DNA transfer is specific for the DNA left of the RB (the T-DNA), rather than for the DNA left of the LB because
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Q. 3) The restriction endonuclease Eco52I recognizes the sequence C/GGCGG and cuts between the first C and the first G, indicated by the slash. DNA cut by which of the following enzymes (given with their recognition sequences and cut sites) could be cloned into a plasmid digested with Eco52I?
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Q. 4) Which one of the following statements about Agrobacterium Ti plasmid is correct?
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Q. 5) A single base pair of DNA weighs 1.1 × 10-21 grams. How many picomoles of a plasmid vector of length 2750 bp are contained in 1 μg of purified DNA?
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Q. 6) Many plasmids have ampr marker. This implies
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Q. 7) T4 polynucleotide kinase is used for
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Q. 8) A gene cannot be isolated from a human genomic DNA library by functional complementation in E. coli because of
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Q. 9) To isolate a gene coding for glucagon, the cDNA library as to be constructed using mRNA isolated from
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Q. 10) Which one of the following cannot be a recognition sequence for a type II restriction enzyme?
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