Methods and Techniques in Applied Biology MCQs | Page - 1

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M

Mr. Dubey • 52.26K Points
Coach 

Q. Which of the following would not possible to address using a Northern Blot?

(A) Location of restriction sites in a particular gene
(B) Spatial expression of a particular gene
(C) Temporal expression of a particular gene
(D) mRNA size
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M

Mr. Dubey • 52.26K Points
Coach 

Q. In southern blotting . . . . . . . . is separated by gel electrophoresis

(A) DNA
(B) m-RNA
(C) t-RNA
(D) Protein
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M

Mr. Dubey • 52.26K Points
Coach 

Q. A restriction enzyme AluI with a 4-bp recognition site produces restriction fragments that should be

(A) 4096 bp
(B) 256 bp
(C) 16 bp
(D) 436 bp
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M

Mr. Dubey • 52.26K Points
Coach 

Q. Terminal transferase is used

(A) To add base at the 3' end of the DNA
(B) To add base at the 5' end of the DNA
(C) To carry out nick translation
(D) To transfer phosphate at the 3' end of the DNA
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M

Mr. Dubey • 52.26K Points
Coach 

Q. Mung bean nuclease could be used for

(A) DNA synthesis
(B) Nucleotide hydrolysis
(C) Trimming single stranded regions in DNA
(D) Removal of phosphate groups from the ends of the DNA
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M

Mr. Dubey • 52.26K Points
Coach 

Q. Many plasmids have Ampicillin marker. This implies

(A) The plasmids contain genes for ampicillin biosynthesis
(B) Ampicillin is required for bacterial growth after transformation
(C) The plasmid contains the gene encoding β-lactamase
(D) Ampicillin is essential for cell survival
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M

Mr. Dubey • 52.26K Points
Coach 

Q. DNA synthesis can be measured by estimating incorporation of radio-labelled

(A) Uracil
(B) Ribose sugar
(C) Thymidine
(D) Adenine
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M

Mr. Dubey • 52.26K Points
Coach 

Q. In agarose gel electrophoresis

(A) DNA migrates towards the negative electrode
(B) Supercoiled plasmids migrate slower than their nicked counterparts
(C) Larger molecules migrate faster than smaller molecules
(D) Ethidium bromide can be used to visualize the DNA
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M

Mr. Dubey • 52.26K Points
Coach 

Q. A cloning vector consisting of CoS site inserted in a plasmid, used to clone DNA fragments of λ phage is

(A) Phage mid
(B) Cosmid
(C) Plasmid
(D) YAC
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M

Mr. Dubey • 52.26K Points
Coach 

Q. Northern blotting is developed by

(A) Ed Southern
(B) Thomas et al
(C) Alwine et al
(D) E. Northern
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