Methods and Techniques in Applied Biology MCQs | Page - 2
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Q. A gene that does not contain introns has an Eco RI site just down stream of the translational termination codon in the 3' untranslated region. The size of the gene is 4 Kbp. The length of 3' UTR is 2 Kbp, the 5' UTR is 1 Kbp and that the open reading frame is 1kbp. Upon probing a southern blot of an Eco RI digested genomic DNA with the radioactively labelled fragment containing only the open reading frame, the size of the band detected by hybridization would be
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Q. Cells deficient in hypoxanthine guanine phospho-ribosyl transferase (HGPRT)
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Q. Which of the following statements gives a correct explanation for the use of vectors containing drug resistance genes in the cloning of recombinant DNA (cDNA) molecules?
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Q. There are two different ways of determining the nucleotide sequence of a nucleic acid: the chemical quencing (Maxam-Gilbert) method and the enzymatic sequencing (Sanger) method. The basic principle/advantage of the Sanger method is
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Q. The ability to clone an adult animal from the nucleus of a differentiated somatic cell proves that
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Q. For protoplast fusion to be successful in plant cells.
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Q. The process used for separation of protein in polyacrylamide gel is called:
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Q. The technique which involves addition or deletions of genes is
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Q. In Southern blotting, DNA is fixed on
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